Classroom Flow Cytometry Training

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Basic to Advanced 

Our classroom based learning programs give you the fundamental knowledge critical to success with flow cytometry. We allow you to select course areas for extra emphasis to ensure maximum value is received. Review the topics below and reach out to us to discuss options and availability.

Continuing Educational Units can be provided at no extra charge.

Fundamentals

  • How a flow cytometer works 

  • Fluorochromes

  • Data Analysis

  • Experimental Design

Advanced

  • Multi-color Flow Cytometry

  • Compensation

  • tSNE/SPADE

  • Cell Sorting

Fundamental Topics


How a flow cytometer works
Learn how a flow cytometer works and the basics of how to use this powerful technology to advance your research.  

Fluorochromes
There are many fluorochromes to choose from depending on your instrument. We teach you how to choose colors based on your instrument and the cells you are working with. You will also learn basic compensation, key to separate the signals from the fluorochromes.

Data Analysis
Learn how to analyze the data generated from a flow cytometry experiment using different types of software. You will learn how to express the data in a meaningful way to understand the biology.

Experimental Design
Learn how to design your experiments and what controls are needed. You will learn how to interpret the data generated from a flow cytometry experiment and how to recognise and troubleshoot issues as they arise.    

Advanced Topics


Multi-color flow cytometry
This technique allows you to look at more than one variable in the cells you are interested in. Because using more than 5 colors in a flow cytometry experiment can be challenging, we teach the best practice approaches and share the expert tips and tricks for setting up multi-color flow experiments.

Compensation
This key tool separates the light from different fluorochromes to allow you to understand the data. Though simple on the surface, mastering compensation of more than 4 colors is challenging.
 
tSNE/SPADE
tSNE (t-distributed stochastic neighbor embedding) and SPADE (spanning-tree progression analysis of density-normalized events) are advanced analysis tools that can help to disseminate very complex data sets.  Our experts help you understand the nuances of transforming high dimensional data into visual clusters.

Cell Sorting
Specific cells can be isolated using a cell sorter. We teach you how sorting works and how to prepare your cells to optimize the sorting quality.